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Image Search Results
Journal: Cancers
Article Title: The RHOA Mutation G17V Does Not Lead to Increased Migration of Human Malignant T Cells but Is Associated with Matrix Remodelling
doi: 10.3390/cancers15123226
Figure Lengend Snippet: ( A , B ) Volume, track speed, track length, and displacement of T-cell lymphoma cell lines expressing RHOA-G17V in native lymphoid tissue. ( A ) Volume of cell bodies (means). Each dot represents the volume of one cell from three independent experiments. * p = 0.039, Mann–Whitney-test. ( B ) Mean track speed of the empty-vector-transfected or RHOA-G17V-overexpressing HuT78 and HH cells compared with the endogenous human-CD3-positive T cells and the CD19-positive B cells. Each dot represents the mean track-based speed of one cell; the cells were recorded in three independent experiments. ** p < 0.01, Kruskal–Wallis test with Dunn´s post-test for multiple comparisons. ( C ) Track length of the empty-vector-transfected or RHOA-G17V-overexpressing HuT78 and HH cells compared with endogenous human-CD3-positive T cells and the CD19-positive B cells. Each dot represents the track length of one cell; the cells were recorded in three independent experiments. * p < 0.05, *** p < 0.001, Kruskal–Wallis test with Dunn´s post-test for multiple comparisons. ( D ) Displacement of the empty-vector-transfected or RHOA-G17V-overexpressing HuT78 and HH cells compared with the endogenous human-CD3-positive T cells and CD19-positive B cells. Each dot represents the track length of one cell; the cells were recorded in three independent experiments. *** p < 0.001, Kruskal–Wallis test with Dunn´s post-test for multiple comparisons. ( E ) Examples of empty-vector-transfected HuT78 cells (green) with reconstructed tracks of a 15 min movie in native lymphoid tissue. ( F ) Examples of RHOA-G17V-expressing HuT78 cells (pink) with reconstructed tracks of a 15 min movie in native lymphoid tissue.
Article Snippet: Endogenous B and T cells were labelled with
Techniques: Expressing, MANN-WHITNEY, Plasmid Preparation, Transfection